4.11 - 1251 ratings - Source

Presents major reviews of all current areas of acarology research.One to 20 mites (depending on size and availability of specimens) were crushed in 400 I¼l extraction buffer (100 mM Tris-HCl pH 8.0, 1.4 M NaCl ... removing the residual 70% ethanol with a pipette, the pellet was dried briefly before resuspending in 20 I¼l TE (Tris pH 8.0 10 mM, EDTA 1 mM). ... Negative controls in which no DNA template was added were always run to check for contamination ofreagents.

Author: DE Walter, R. B. Halliday, H Proctor, RA Norton
Publisher:CSIRO PUBLISHING - 2001-07-16

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